A-A-340 Insecticide; Mixture Of D-Trans Allethrin And ResmethrinThis commercial item description covers an insecticide space spray for use in enclosed areas against mosquitoes, flies, gnats, and other small flying insects; it may be sprayed directly on insects.

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1. Apparatus- gas chromatograph equipped with flame ionization detector and suitable integrator. Conditions may be modified, if necessary, to obtain proper separations, reproducibility, and sensitivity. Operating conditions shall be reported as a part of the test report.

Recommended Chromatographic Conditions

CHARACTERISTICS	CONDITION
Column Material	Glass
Column Dimensions	2 m X 4 mm. I.D.
Column Support	80/100 mesh Chromosorb W(HP)
Liquid Phase	5% SE-30
Injector Temperature	250^oC
Column Temperature	220^oC for 10 min. and then programmed to 260^oC at 10^oC/min.
Detector Temperature	250^oC
Carrier Gas	Helium
Carrier Gas Flow Rate	30 ml per min.

2. Internal Standard Solution- Dissolve 1 gram docosane in chloroform, transfer to a 200 ml volumetric flask and dilute to the mark with chloroform.

3. Standard Solution- Transfer about 30 mg of analytical standard resmethrin and 25 mg d-trans allethrin accurately weighed to a 10-ml volumetric flask and dissolve in about 2 ml chloroform. Add 5.0 ml of the internal standard solution, dilute to the volume with chloroform and mix.

3.1 Analytical standard resmethrin may be obtained from S.B. Penick & Co., Commercial Development- Pesticides, 215 Watchung Avenue, Orange, NJ 07050. Analytical Standard d-Trans Allethrin may be obtained from MoLaughin, Gormly, King Co., 8810 Tenth Avenue North, Minneapolis, MN 55427.

4. Sample Preparation- Weigh aerosol can place in a dry ice-acetone bath or freezer until contents solidify. Remove container from bath, punch two small holes in the bottom, and allow propellant to evaporate in a fume hood. Do not apply extra heat. Quantitatively transfer the liquid to a 500-ml volumetric flask, rinse the can with water and dilute to volume. Pipette a 25 ml aliquot into a 100 ml round bottom flask and remove the water using a rotary evaporator at 60^oC under vacuum.